Khuda-Bukhsh Anisur Rahman
Dr. Anisur Rahman Khuda-Bukhsh
Emeritus Fellow, UGC, Department of Zoology, University of Kalyani,
Homeopathic Secalecor 30C demonstrates regression of induced skin papilloma in mice: the possible mechanism of action
We demonstrated abilities of various homeopathic remedies (Chelidoniummajus, Condurango, Pneumusboldus and many others), mother tinctures and ultra-high dilutions (UHD), to have anticancer potential, both in vivo and in vitro. In this paper, we would highlight briefly ability of Secalecor 30C (diluted 1060 times) to ameliorate DMBA+ croton oil- induced skin papilloma and elucidate the possible signalling mechanism involved in the process. Sec cor 30C intervened successfully in protection/regression of papilloma in treated mice as evidenced by histological, cytogenetical, immunofluorescence, ELISA and immunoblot findings. Critical analysis of several signal proteins like AhR, PCNA, Akt, Bcl-2, Bcl-xL, NF-κB and IL-6 and of pro-apoptotic proteins like cytochrome c, Bax, Bad, Apaf, caspase-3 and -9 revealed that Sec cor 30C suitably modulated their expression levels along with ameliorative changes in skin papilloma. FACS data also revealed an increase of cell population at S and G2 phases and decrease in sub-G1 and G1 phases in carcinogen-treated placebo-fed mice, but these were found to be near normal in the Sec cor 30C-fed mice. There was reduction in genotoxic and DNA damages in bone marrow cells of Sec cor 30C fed mice, as revealed from data of cytogenetic and Comet assays. Changes in histological features of skin papilloma were noted. Immunofluorescence studies of AhR and PCNA also suggested reduced expression of these proteins in Sec cor 30C fed mice. Furthermore, this study also supports our “gene-regulatory hypothesis” that potentized homeopathic drugs act at gene regulatory levels.
Nano-encapsulation of and nanoprecipitation by homeopathic mother tinctures: Characterization, drug-DNA interaction, biodistribution of homeopathic nanomedicines and their signalling pathways in understanding molecular mechanism of biological action
We prepared dried extracts of homeopathic mother tinctures like Gelsemium semperverens, Ruta graveolens, Phytolacca decandra, Chelidonium majus etc, nanoencapsulated them with non-toxic and biodegradable polymers of poly(lactide-co-gycolide) and characterized these homeopathic nano-capsules for their size, zeta potential, surface morphology, entrapment efficiency etc with the aid of dynamic light scattering, electron microscopies (scanning, transmission and high-resolution), atomic force and confocal microscopies, fourier transform infrared spectroscopy, etc. Biological action of the different types of nanoparticles produced by loading different extracts, mostly measuring around 100 nM, was then tested, mainly in some cancer and normal cells in vitro and in mice in vivo. Anticancer potential of PLGA-loaded nanoparticles, if any, was compared with that of their respective mother tinctures. The nano-forms always showed faster cellular entry and showed strikingly enhanced biological efficacy at a much reduced dose. Further, when their tissue distribution in mice in vivo was studied, the presence of the nanocapsules could be detected in many tissues including the brain, indicating their ability to cross blood brain barrier. We also examined if they could interact with DNA and make conformational changes in DNA by circular dichroism spectroscopy and by determining and comparing their melting temperature profiles.They demonstrated ability to modulate signalling pathways in a favourable way in cancer. Efficacy of some of their principal biologically active ingredients and their respective nanoforms were also compared. We were the first to nano-precipitate silver from silver nitrate solution by different homeopathic mother tinctures and examine their biological action against some cancer and normal cells. These silver nanoparticles showed subtle differences in respect of their physico-chemical characters, and their biological activities. These findings can link the recent reports on discovery of nanoparticles in ultra-dilutions of homeopathic remedies and their role in biological action, giving further credence to our gene regulatory hypothesis.
Dr. Khuda-Bukhsh has been a faculty member at University of Kalyani, Deptt. of Zoology since April 1975. In 2013 he retired as Professor of Zoology serving as Emeritus Fellow of UGC since 2015. He has guided doctoral research on numerous homeopathy research topics. Widely travelled Dr. Khuda-Bukhsh presented research papers in many countries, chaired many scientific sessions in National and International Conferences, Symposia and Seminars. He has published more than 300 research papers, reviews and book chapters in Indian and International peer-reviewed journals of repute. On research related to homeopathy over one hundred papers so far published. Gene regulatory hypothesis and homeopathic nano-medicines of special significance. Homeopathy research works included in international documentary film “The Devil’s Water” shown in several European and American TV and News channels. He has received among others the international Je Ma award from Korean Society of Pharmacopuncture for outstanding research paper presented in Stockholm in 2013. He has handled many scientific projects- nationaly (ICAR, UGC, CSIR, AYUSH, DOE, etc.) and internationayl (Rayne Institute/ UK and Boiron Lab/ France). Excerpts of some of his published research papers were translated and published in several European and Asian languages.